Microenvironment and Immunology Quantitative and Functional Alterations of Plasmacytoid Dendritic Cells Contribute to Immune Tolerance in Ovarian Cancer

نویسندگان

  • Sana Intidhar Labidi-Galy
  • Vanja Sisirak
  • Pierre Meeus
  • Michael Gobert
  • Isabelle Treilleux
  • Agathe Bajard
  • Jean-Damien Combes
  • Julien Faget
  • François Mithieux
  • Alexandre Cassignol
  • Olivier Tredan
  • Isabelle Durand
  • Christophe Caux
  • Jean-Yves Blay
  • Nathalie Bendriss-Vermare
چکیده

In ovarian cancer, the immune system fails to eradicate established tumors partly due to the induction of immune tolerance within tumor microenvironment. In this study, we investigated the contribution of plasmacytoid dendritic cells (pDC) in the establishment of immune tolerance in a cohort of 44 ovarian cancer patients. In the tumor and malignant ascites, CD4þCD123þBDCA2þ pDC were the most abundant dendritic cell subset; however, they were profoundly depleted in peripheral blood. The presence of pDC in primary ovarian cancer, but not ascites, was an independent prognostic factor associated with early relapse. Following chemotherapy, we observed a partial restoration of blood pDC levels in patients in complete remission. These findings show preferential recruitment of pDC into tumors where they express a partially mature phenotype that may reflect an in situ activation. Importantly, compared with pDC found in ascites or blood, tumor-associated pDC (TApDC) produced less IFN-a, TNF-a, IL-6, macrophage inflammatory protein-1b, and RANTES in response to toll-like receptor stimulation, and alterations in pDC functions were mainly mediated through tumor-derived TNF-a and TGF-b. Unlike ascites-derived pDC, TApDC induced IL-10 production from allogeneic naive CD4þ T lymphocytes, suggesting the existence of a paracrine immunosuppressive loop. Taken together, our findings indicate that both local and systemic dysfunction of pDC play a critical role in the progression of ovarian cancer via induction of immune tolerance. Cancer Res; 71(16); 5423–34. 2011 AACR.

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تاریخ انتشار 2011